rabbit anti fabp6  (Biorbyt)

Journal: medRxiv

Article Title: Multiomic analysis reveals cellular and epigenetic plasticity in intestinal pouches of ulcerative colitis patients

doi: 10.1101/2023.11.11.23298309

Figure Lengend Snippet: ( A ) Representative immunohistochemistry images from pre-pouch and pouch biopsies for H&E, FABP6, CEACAM5, CA2 staining. ( B ) Histological enteritis score of pre-pouch and pouch samples. ( C ) Average gene expression in the bulk RNA-seq data from healthy pouch patients. ( D ) Average gene expression in the bulk RNA-seq data from patients with actively inflammatory pouches. The expressions in panel C and D are scaled in each column including prepouch and pouch. ( E ) The deconvolved cell type proportions in healthy pouches from bulk RNA-seq data. ( F ) The deconvolved cell type proportions in inflammatory pouches from bulk RNA-seq data.

Article Snippet: Primary antibodies used were rabbit anti-APOA4 (1:1,000, Sigma HPA001352), rabbit anti-FABP6 (1:200, Sigma HPA012601), mouse anti-CA2 (Santa Cruz Biotechnology sc-48351), and mouse anti-CEACAM5 (R&D Systems MAB41281).

Techniques: Immunohistochemistry, Staining, Expressing, RNA Sequencing Assay

Journal: Cells

Article Title: Mechanism of Dyslipidemia in Obesity—Unique Regulation of Ileal Villus Cell Brush Border Membrane Sodium–Bile Acid Cotransport

doi: 10.3390/cells8101197

Figure Lengend Snippet: Na–bile acid cotransport expression in human distal ileal villus cells. ASBT expression was significantly increased in villus cells from obese human ileum compared to normal BMI human ileum.

Article Snippet: The following antibodies were used to detect the expression of the specific proteins: Farnesoid X receptor: FXR, sc-25309, Santa Cruz Biotechnology, Inc., Dallas, TX, USA [ , ]; ileal bile-acid-binding protein: ILBABP, AP26370PU-N, OriGene Global, Rockville, MD, USA [ ]; organic solute transporter alpha: OSTα, SAB1306154, Sigma-Aldrich Corporation, St. Louis, MO, USA; and Ezrin: MA5-13862, Invitrogen Life Technologies, Carlsbad, CA, USA [ , ].

Techniques: Expressing

Journal: Cells

Article Title: Mechanism of Dyslipidemia in Obesity—Unique Regulation of Ileal Villus Cell Brush Border Membrane Sodium–Bile Acid Cotransport

doi: 10.3390/cells8101197

Figure Lengend Snippet: FXR and bile acid handling protein expression in Zucker rat villus cells. ( A ) FXR expression was significantly increased in ileal villus cells from OZR compared to LZR. ( B ). Ileal bile acid binding protein levels were also significantly increased in villus cells from OZR compared to leans. ( C ) Organic solute transporter alpha (OSTα) protein expression was significantly increased in villus cells from OZR compared to LZR. ( D ) OSTα protein expression was also increased in villus cell plasma membrane from OZR compared to LZR.

Article Snippet: The following antibodies were used to detect the expression of the specific proteins: Farnesoid X receptor: FXR, sc-25309, Santa Cruz Biotechnology, Inc., Dallas, TX, USA [ , ]; ileal bile-acid-binding protein: ILBABP, AP26370PU-N, OriGene Global, Rockville, MD, USA [ ]; organic solute transporter alpha: OSTα, SAB1306154, Sigma-Aldrich Corporation, St. Louis, MO, USA; and Ezrin: MA5-13862, Invitrogen Life Technologies, Carlsbad, CA, USA [ , ].

Techniques: Expressing, Binding Assay, Clinical Proteomics, Membrane